| First Author | Fang Q | Year | 2013 |
| Journal | FEBS Lett | Volume | 587 |
| Issue | 1 | Pages | 67-72 |
| PubMed ID | 23178715 | Mgi Jnum | J:192579 |
| Mgi Id | MGI:5465395 | Doi | 10.1016/j.febslet.2012.11.002 |
| Citation | Fang Q, et al. (2013) Juxtamembrane tryptophans of synaptobrevin 2 control the process of membrane fusion. FEBS Lett 587(1):67-72 |
| abstractText | Synaptobrevin 2 (Syb2), syntaxin (Sx1A), and SNAP-25, generate a force to induce fusion pore formation. The v-SNARE, Syb2, is anchored to the vesicle membrane by a single transmembrane domain. Here we show that 2 tryptophans (W89/W90) located in the juxtamembrane domain of Syb2, which stabilize the transmembrane (TM) domain position, control the ratio of spontaneous vs. stimulated membrane fusion events in chromaffin cells. Changing the 2 hydrophobic tryptophans to neutral alanines promotes spontaneous membrane fusion, faster transmitter release kinetics and complete release from individual vesicles. The results indicate that the two tryptophans act as a fusion clamp making fusion stimulus-dependent. |
