| First Author | Kalitsis P | Year | 2000 |
| Journal | Genes Dev | Volume | 14 |
| Issue | 18 | Pages | 2277-82 |
| PubMed ID | 10995385 | Mgi Jnum | J:64874 |
| Mgi Id | MGI:1890069 | Doi | 10.1101/gad.827500 |
| Citation | Kalitsis P, et al. (2000) Bub3 gene disruption in mice reveals essential mitotic spindle checkpoint function during early embryogenesis. Genes Dev 14(18):2277-82 |
| abstractText | Bub3 is a conserved component of the mitotic spindle assembly complex. The protein is essential for early development in Bub3 gene-disrupted mice, evident from their failure to survive beyond day 6.5-7.5 postcoitus (pc). Bub3 null embryos appear normal up to day 3.5 pc but accumulate mitotic errors from days 4.5-6.5 pc in the form of micronuclei, chromatin bridging, lagging chromosomes, and irregular nuclear morphology. Null embryos treated with a spindle-depolymerising agent fail to arrest in metaphase and show an increase in mitotic disarray. The results confirm Bub3 as a component of the essential spindle checkpoint pathway that operates during early embryogenesis. |
