| First Author | Shah HB | Year | 2012 |
| Journal | J Leukoc Biol | Volume | 92 |
| Issue | 4 | Pages | 883-93 |
| PubMed ID | 22798686 | Mgi Jnum | J:189639 |
| Mgi Id | MGI:5446585 | Doi | 10.1189/jlb.0412177 |
| Citation | Shah HB, et al. (2012) Type II NKT cells facilitate Alum-sensing and humoral immunity. J Leukoc Biol 92(4):883-93 |
| abstractText | Alum-based adjuvants facilitate vaccine-driven humoral immunity, but their mechanism of action remains poorly understood. Herein, we report that lack of type II NKT cells is associated with intact, mature B cells but dampened humoral immunity following immunization with Alum-adsorbed T-dependent antigen. Type II NKT cells facilitated production of IL-4, IL-5, IL-10, IL-13, and antibody by LN and splenocyte cultures following Alum/antigen administration in vivo and antigen restimulation in vitro. Addition of IL-4 and IL-5 to type II NKT-deficient cultures restored in vitro antibody production. Intracellular staining revealed that Alum-primed type II NKT cells coordinated IL-4 secretion by T cells. Alum did not significantly affect CD1d expression in vivo, but addition of CD1d-blocking mAb diminished cytokine production and in vitro antibody production. Type II NKT cells therefore function as part of the Alum-sensing apparatus and in a CD1d-dependent manner, facilitate T(H)2-driven humoral immunity. This may have important consequences for understanding the mechanism of action of Alum-containing vaccines. |
