| First Author | Bakocević N | Year | 2010 |
| Journal | J Immunol | Volume | 184 |
| Issue | 3 | Pages | 1317-27 |
| PubMed ID | 20042584 | Mgi Jnum | J:159503 |
| Mgi Id | MGI:4443183 | Doi | 10.4049/jimmunol.0902277 |
| Citation | Bakocevic N, et al. (2010) T cell-dendritic cell interaction dynamics during the induction of respiratory tolerance and immunity. J Immunol 184(3):1317-27 |
| abstractText | Dendritic cells (DCs) residing in the lung are known to acquire inhaled Ag and, after migration to the draining bronchial lymph node (brLN), to present it to naive T cells in an either tolerogenic or immunogenic context. To visualize endogenous lung-derived DCs, we applied fluorescent latex beads (LXs) intratracheally, thereby in vivo labeling the majority of phagocytic cells within the lung. Of note, LX-bearing cells subsequently arriving in the draining brLN were found to represent lung-derived migratory DCs. Imaging explanted brLN by two-photon laser-scanning microscopy, we quantitatively analyzed the migration and interaction behavior of naive CD4(+) T cells and endogenous, lung-derived DC presenting airway-delivered Ag under inflammatory or noninflammatory conditions. Ag-specific naive CD4(+) T cells engaged in stable as well as transient contacts with LX-bearing DCs in both situations and displayed similar overall motility kinetics, including a pronounced decrease in motility at 16-20 h after antigenic challenge. In contrast, the comparative analysis of T cell-DC cluster sizes as well as contact durations strongly suggests that lung-derived migratory DCs and naive CD4(+) T cells form more stable, long-lasting contacts under inflammatory conditions favoring the induction of respiratory immunity. |
