| First Author | Wu CA | Year | 2002 |
| Journal | Arterioscler Thromb Vasc Biol | Volume | 22 |
| Issue | 8 | Pages | 1347-53 |
| PubMed ID | 12171799 | Mgi Jnum | J:103215 |
| Mgi Id | MGI:3608741 | Doi | 10.1161/01.atv.0000026297.50542.62 |
| Citation | Wu CA, et al. (2002) Cholesteryl ester transfer protein expressed in lecithin cholesterol acyltransferase-deficient mice. Arterioscler Thromb Vasc Biol 22(8):1347-53 |
| abstractText | OBJECTIVE: Regulation of plasma cholesteryl ester transfer protein (CETP) concentration was studied in lecithin-cholesterol acyltransferase (LCAT)-knockout mice. METHODS AND RESULTS: LCAT-knockout mice were cross-bred with CETP transgenic mice. The offspring (n=63) were classified for LCAT genotype and plasma CETP levels (no CETP, low CETP, and high CETP). High density lipoprotein (HDL) decreased as LCAT decreased in each CETP-level group. In the lcat(+/+) and lcat(+/-) mice, plasma CETP varied from 0 to 30 micro g/mL, whereas it was <10 micro g/mL in the lcat(-/-) mice. HDL cholesterol and phospholipid decreased and HDL triglyceride and apolipoprotein B increased in CETP in the lcat(+/+) and lcat(+/-) mice, whereas there was no difference in HDL between low and high CETP. An effect of CETP on HDL was not detected in the lcat(-/-) mice because of the absence of mature HDL. Genomic DNA and mRNA of CETP were correlated and were similar in the lcat(-/-) and lcat(+/+) mice. Plasma CETP was correlated with its genomic DNA and mRNA, but the slope of the increase was much lower in the lcat(-/-) mice. Whereas plasma CETP mostly associates with HDL in the lcat(+/+) mouse, it is found free in the lcat(-/-) mouse. CONCLUSIONS: Plasma CETP is posttranscriptionally downregulated in the lcat(-/-) mice, presumably by its extremely low HDL. |
