| Primary Identifier | MGI:2180700 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Cd22 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd-Hprt1<b-m3> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A PGK-neo cassette replaced a portion of the 5' untranslated region, the leader sequence, and a portion of the exon encoding the immunoglobulin (Ig) 1 domain. A stop codon was introduced into the 5' end of the exon encoding the Ig 1 domain. Immunohistochemical staining of frozen spleen sections, using various Ig-specific antibodies, showed a lack of secreted, cell surface, and cytoplasmic protein. The absence of sell surface expression was further confirmed by immunofluorescence staining of lymphocytes. |
