| Primary Identifier | MGI:2180895 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Irx4 |
| Transmission | Germline | Strain of Origin | 129X1/SvJ |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A PGK-neo construct replaced exon 2 and a portion of exon 3. The targeted allele was shown to produce two truncated transcripts via Northern blot and RT-PCR analyses. Further RT-PCR analysis using various probes showed that the aberrant transcripts lacked the essential ribosome binding domain as well as the initiation codon. |