Primary Identifier | MGI:2180804 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Cd7 |
Transmission | Germline | Strain of Origin | 129 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Exon 3 was disrupted by the introduction of a stop codon upstream of sequence encoding the transmembrane domain. A PGK-neo cassette was inserted just downstream of the introduced stop codon. Northern blot analysis of thymocytes and splenocytes showed a lack of protein expression in homozygous mutant mice. These results were confirmed by RT-PCR analysis. |