| Primary Identifier | MGI:2181025 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Slc12a5 |
| Transmission | Germline | Strain of Origin | 129X1/SvJ |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exon 5 was replaced with a neomycin selection cassette, introducing a frameshift mutation. The deleted region encoded a portion of the second transmembrane domain. Northern analysis revealed a lack of transcript in homozygous mutant mice. Absence of a truncated protein product was confirmed by Western blot analysis using an antibody raised against the N-terminal. |
