| Primary Identifier | MGI:2181521 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Cenpb |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Stop codons were introduced into all three reading frames of the intronless gene by the insertion a translation frameshift linker into the amino-terminal centromere DNA-binding domain. An IRES-neomycin selection marker was inserted in the 3' untranslated region upstream of the poly adenylation signal. RT-PCR analysis of heterozygous mutant mice showed the expression of a truncated transcript. Mice possessing both this allele and Cenpbtm2Khac were shown to lack transcript and protein was not detected by immunofluorescence staining of metaphase chromosomes. |
