| Primary Identifier | MGI:2181624 | Allele Type | Targeted |
| Attribute String | Hypomorph | Gene | Kit |
| Transmission | Germline | Strain of Origin | 129S4/SvJae |
| Is Recombinase | false | Is Wild Type | false |
| description | This ES cell line contained a cre recombinase gene under the control of the protamine-1 promoter, which is active in male haploid germ cells. |
| molecularNote | An engineered point mutation in codon 719 resulted in a tyrosine to phenylalanine substitution (Y719F). Western blot analysis showed the level of mutant protein in homozygous mutant mice to be similar to that of the wild-type protein in wild-type mice. PI 3'-kinase activity was determined to be reduced by approximately 90% via assays of the PI 3'-kinase dependent activation of Akt. |
