| First Author | Mizutani T | Year | 2015 |
| Journal | Mol Cell Endocrinol | Volume | 408 |
| Pages | 133-7 | PubMed ID | 25463758 |
| Mgi Jnum | J:223654 | Mgi Id | MGI:5660041 |
| Doi | 10.1016/j.mce.2014.11.019 | Citation | Mizutani T, et al. (2015) Identification of novel steroidogenic factor 1 (SF-1)-target genes and components of the SF-1 nuclear complex. Mol Cell Endocrinol 408:133-7 |
| abstractText | Steroidogenic factor 1 (SF-1) is a master regulator of adrenal and reproductive development and function. Although SF-1 was identified as a transcriptional regulator for steroid metabolic enzymes, it has been shown that SF-1 also regulates other genes that are involved in various cellular processes. Previously, we showed that introduction of SF-1 into mesenchymal stem cells resulted in the differentiation of these cells to the steroidogenic lineage. By using this method of differentiation, we performed comprehensive analyses to identify the novel SF-1-target genes and components of the SF-1 nuclear complex. Genome-wide analyses with promoter tiling array and DNA microarray identified 10 genes as novel SF-1-target genes including glutathione S-transferase A family, 5-aminolevulinic acid synthase 1 and ferredoxin reductase. Using SF-1 immuno-affinity chromatography of nuclear proteins followed by MS/MS analysis, we identified 24 proteins including CCAAT/enhancer-binding protein beta as components of SF-1 nuclear complex. In this review, we will describe novel roles of the newly identified genes for steroidogenesis. |
