| First Author | Stoilova B | Year | 2013 |
| Journal | PLoS One | Volume | 8 |
| Issue | 6 | Pages | e65169 |
| PubMed ID | 23755188 | Mgi Jnum | J:203204 |
| Mgi Id | MGI:5525186 | Doi | 10.1371/journal.pone.0065169 |
| Citation | Stoilova B, et al. (2013) Lymphoid to myeloid cell trans-differentiation is determined by C/EBPbeta structure and post-translational modifications. PLoS One 8(6):e65169 |
| abstractText | The transcription factor C/EBPbeta controls differentiation, proliferation, and functionality of many cell types, including innate immune cells. A detailed molecular understanding of how C/EBPbeta directs alternative cell fates remains largely elusive. A multitude of signal-dependent post-translational modifications (PTMs) differentially affect the protean C/EBPbeta functions. In this study we apply an assay that converts primary mouse B lymphoid progenitors into myeloid cells in order to answer the question how C/EBPbeta regulates (trans-) differentiation and determines myeloid cell fate. We found that structural alterations and various C/EBPbeta PTMs determine the outcome of trans-differentiation of lymphoid into myeloid cells, including different types of monocytes/macrophages, dendritic cells, and granulocytes. The ability of C/EBPbeta to recruit chromatin remodeling complexes is required for the granulocytic trans-differentiation outcome. These novel findings reveal that PTMs and structural plasticity of C/EBPbeta are adaptable modular properties that integrate and rewire epigenetic functions to direct differentiation to diverse innate immune system cells, which are crucial for the organism survival. |
