| Primary Identifier | MGI:2182328 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Cyp7b1 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exon 2 was disrupted by the recombination of a construct containing IRES-lacZ and neo genes. Normal transcript was undetected by Northern blot analysis of homozygous mutant mice. Individual assays of various tissue homogenates showed a lack of DHEA hydroxylation, indicating the functional ablation of the encoded protein in homozygous mutant mice. The results obtained from beta-galactose staining differed from those of in situ hybridization. |
