| First Author | Horikawa S | Year | 2015 |
| Journal | Sci Rep | Volume | 5 |
| Pages | 17948 | PubMed ID | 26639755 |
| Mgi Jnum | J:250501 | Mgi Id | MGI:6102751 |
| Doi | 10.1038/srep17948 | Citation | Horikawa S, et al. (2015) PDGFRalpha plays a crucial role in connective tissue remodeling. Sci Rep 5:17948 |
| abstractText | Platelet derived growth factor (PDGF) plays a pivotal role in the remodeling of connective tissues. Emerging data indicate the distinctive role of PDGF receptor-alpha (PDGFRalpha) in this process. In the present study, the Pdgfra gene was systemically inactivated in adult mouse (alpha-KO mouse), and the role of PDGFRalpha was examined in the subcutaneously implanted sponge matrices. PDGFRalpha expressed in the fibroblasts of Pdgfra-preserving control mice (Flox mice), was significantly reduced in the sponges in alpha-KO mice. Neovascularized areas were largely suppressed in the alpha-KO mice than in the Flox mice, whereas the other parameters related to the blood vessels and endothelial cells were similar. The deposition of collagen and fibronectin and the expression of collagen 1a1 and 3a1 genes were significantly reduced in alpha-KO mice. There was a significantly decrease in the number and dividing fibroblasts in the alpha-KO mice, and those of macrophages were similar between the two genotypes. Hepatocyte growth factor (Hgf) gene expression was suppressed in Pdgfra-inactivated fibroblasts and connective tissue. The findings implicate the role of PDGFRalpha-dependent ECM and HGF production in fibroblasts that promotes the remodeling of connective tissue and suggest that PDGFRalpha may be a relevant target to regulate connective tissue remodeling. |
