| Primary Identifier | MGI:2182810 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Ppef1 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The last 21 codons of exon 5 were replaced with a lacZ-neo cassette via homologous recombination and results in an in-frame fusion of the lacZ gene to exon 5. Gene expression was absent in retinas and brain stem of homozygous mutant animals as determined by RT-PCR analysis. Beta-galactosidase activity was not present in the mutant animals. |
