| Primary Identifier | MGI:2182610 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Ung |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The insertion of a neomycin selection cassette disrupted exon 4, which encodes residues of the uracil-binding pocket. The insertion was designed to impair the production of both nuclear and mitochondrial isoforms. Uracil-DNA glycosylase activity was shown to be markedly reduced in cell extracts from homozygous mutant mice. The residual activity was not inhibited by antibodies raised against the encoded protein. |
