Primary Identifier | MGI:2182626 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Efnb2 |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The locus was disrupted by the insertion of a construct, containing a floxed neo gene and a lacZ gene fused to the transmembrane domain of the mouse Ntrk2 gene. The insertion was made just downstream of the sequences encoding the signal peptide. RT-PCR analysis confirmed the absence of transcript in homozygous mutant mice. Staining of heterozygous embyros showed expression of beta-galactosidase. |