| Primary Identifier | MGI:2182819 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Mark2 |
| Transmission | Germline | Strain of Origin | 129X1/SvJ |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The insertion of a neomycin selection cassette deleted sequence from exons 2, 3, and 4. The replaced region encoded residues of the glycine-rich motif involved in MgATP-binding. Western blot analysis of extracts from fibroblasts showed an absence of all known isoforms produced from the targeted allele in homozygous mutant embryos. Immunoprecipitates of tissue extracts derived from homozygous mutant mice failed to phosphorylate GST-Cdc25C in vitro, incdicating ablation of kinase activity. |
