Primary Identifier | MGI:2183284 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | Pten |
Transmission | Germline | Strain of Origin | Not Specified |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A targeting vector which contained a floxed neomycin selection cassette in intron 4 and a loxP site in intron 5 was electroporated into ES cells that were subsequently transiently transfected with a cre recombinase expression plasmid. The expression of cre resulted in the excision of the neo transgene, leaving exon 5 flanked by loxP sites. Mice carrying this allele were used to generate Ptentm1.1. |