| Primary Identifier | MGI:2182568 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Ddx4 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A 4.5 kb region of the gene spanning exons 9 and 10 encoding the ATPase domain was replaced with a IRES-lacZ-PGK-neo cassette via homologous recombination. No gene transcripts were detected by RT-PCR analysis using primers amplifying exon 8 or 11, and Western blot analysis showed complete absence of protein product from testes of homozygous mutants. |