Primary Identifier | MGI:2182720 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Gadd45a |
Transmission | Germline | Strain of Origin | 129 |
Is Recombinase | false | Is Wild Type | false |
description | ES cell line = ESVJ-1183 (129X1/SvJ) or HM-1 (129P2/OlaHsd). |
molecularNote | 2.5 kb of the promoter and exons 1-3 were replaced with a PGK-neo cassette via homologous recombination. Absence of gene expression in homozygous mutants was verified by RNase protection assay using an exon 3 probe and mouse embryonic fibroblasts derived from E13.5 homozygous mutant embryos. |