| First Author | Koike T | Year | 2019 |
| Journal | Elife | Volume | 8 |
| PubMed ID | 31225793 | Mgi Jnum | J:278553 |
| Mgi Id | MGI:6355607 | Doi | 10.7554/eLife.44245 |
| Citation | Koike T, et al. (2019) The quantity of CD40 signaling determines the differentiation of B cells into functionally distinct memory cell subsets. Elife 8:e44245 |
| abstractText | In mice, memory B (Bmem) cells can be divided into two subpopulations: CD80(hi) Bmem cells, which preferentially differentiate into plasma cells; and CD80(lo) Bmem cells, which become germinal center (GC) B cells during a recall response. We demonstrate that these distinct responses can be B-cell-intrinsic and essentially independent of B-cell receptor (BCR) isotypes. Furthermore, we find that the development of CD80(hi) Bmem cells in the primary immune response requires follicular helper T cells, a relatively strong CD40 signal and a high-affinity BCR on B cells, whereas the development of CD80(lo) Bmem cells does not. Quantitative differences in CD40 stimulation were enough to recapitulate the distinct B cell fate decisions in an in vitro culture system. The quantity of CD40 signaling appears to be translated into NF-kappaB activation, followed by BATF upregulation that promotes Bmem cell differentiation from GC B cells. |
