| First Author | Xu T | Year | 2019 |
| Journal | Front Immunol | Volume | 10 |
| Pages | 184 | PubMed ID | 30828328 |
| Mgi Jnum | J:294340 | Mgi Id | MGI:6445282 |
| Doi | 10.3389/fimmu.2019.00184 | Citation | Xu T, et al. (2019) NFAT1 and NFAT2 Differentially Regulate CTL Differentiation Upon Acute Viral Infection. Front Immunol 10:184 |
| abstractText | CD8(+) T cell differentiation orchestrated by transcription regulators is critical for balancing pathogen eradication and long-term immunity by effector and memory CTLs, respectively. The transcription factor Nuclear Factor of Activated T cells (NFAT) family members are known for their roles in T cell development and activation but still largely undetermined in CD8(+) T cell differentiation in vivo. Here, we interrogated the role of two NFAT family members, NFAT1 and NFAT2, in the effector and memory phase of CD8(+) T cell differentiation using LCMV(Arm) acute infection model. We found that NFAT1 is critical for effector population generation whereas NFAT2 is required for promoting memory CTLs in a cell intrinsic manner. Moreover, we found that mice lacking both NFAT1 and NFAT2 in T cells display a significant increase in KLRG1(hi) CD127(hi) population and are unable to clear an acute viral infection. NFAT-deficient CTLs showed different degrees of impaired IFN-gamma and TNF-alpha expression with NFAT1 being mainly responsible for IFN-gamma production upon ex-vivo stimulation as well as for antigen-specific cytotoxicity. Our results suggest that NFAT1 and NFAT2 have distinct roles in mediating CD8(+) T cell differentiation and function. |
