Primary Identifier | MGI:2182939 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Mtf1 |
Transmission | Germline | Strain of Origin | 129/Sv |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A neomycin selection cassette replaced sequences encoding the first two and a portion of the third zinc-finger domain. RT-PCR analysis of RNA from mutant embryos revealed the presence of an amino-terminal fragment, truncating 39 base pairs downstream of the 5' insertion point. The truncated transcript lacks sequence encoding 3 zinc-finger domains and the acidic, proline-rich, and serine/threonine-rich regions. |