| First Author | McFarland AP | Year | 2021 |
| Journal | Immunity | PubMed ID | 33945787 |
| Mgi Jnum | J:305787 | Mgi Id | MGI:6706539 |
| Doi | 10.1016/j.immuni.2021.03.024 | Citation | McFarland AP, et al. (2021) Multi-tissue single-cell analysis deconstructs the complex programs of mouse natural killer and type 1 innate lymphoid cells in tissues and circulation. Immunity |
| abstractText | Natural killer (NK) cells and type 1 innate lymphoid cells (ILC1s) are heterogenous innate lymphocytes broadly defined in mice as Lin(-)NK1.1(+)NKp46(+) cells that express the transcription factor T-BET and produce interferon-gamma. The ILC1 definition primarily stems from studies on liver and small intestinal populations. However, NK1.1(+)NKp46(+) cells in the salivary glands, uterus, adipose, and other tissues exhibit nonuniform programs that differ from those of liver or intestinal ILC1s or NK cells. Here, we performed single-cell RNA sequencing on murine NK1.1(+)NKp46(+) cells from blood, spleen, various tissues, and solid tumors. We identified gene expression programs of tissue-specific ILC1s, tissue-specific NK cells, and non-tissue-specific populations in blood, spleen, and other tissues largely corresponding to circulating cells. Moreover, we found that circulating NK cell programs were reshaped in tumor-bearing mice. Core programs of circulating and tumor NK cells paralleled conserved human NK cells signatures, advancing our understanding of the human NK-ILC1 spectrum. |
