| First Author | Tsujino K | Year | 2017 |
| Journal | Am J Physiol Lung Cell Mol Physiol | Volume | 312 |
| Issue | 1 | Pages | L22-L31 |
| PubMed ID | 27864286 | Mgi Jnum | J:238723 |
| Mgi Id | MGI:5823514 | Doi | 10.1152/ajplung.00428.2016 |
| Citation | Tsujino K, et al. (2017) Transforming growth factor-beta plays divergent roles in modulating vascular remodeling, inflammation, and pulmonary fibrosis in a murine model of scleroderma. Am J Physiol Lung Cell Mol Physiol 312(1):L22-L31 |
| abstractText | The efficacy and feasibility of targeting transforming growth factor-beta (TGFbeta) in pulmonary fibrosis and lung vascular remodeling in systemic sclerosis (SSc) have not been well elucidated. In this study we analyzed how blocking TGFbeta signaling affects pulmonary abnormalities in Fos-related antigen 2 (Fra-2) transgenic (Tg) mice, a murine model that manifests three important lung pathological features of SSc: fibrosis, inflammation, and vascular remodeling. To interrupt TGFbeta signaling in the Fra-2 Tg mice, we used a pan-TGFbeta-blocking antibody, 1D11, and Tg mice in which TGFbeta receptor type 2 (Tgfbr2) is deleted from smooth muscle cells and myofibroblasts (alpha-SMA-CreER;Tgfbr2flox/flox). Global inhibition of TGFbeta by 1D11 did not ameliorate lung fibrosis histologically or biochemically, whereas it resulted in a significant increase in the number of immune cells infiltrating the lungs. In contrast, 1D11 treatment ameliorated the severity of pulmonary vascular remodeling in Fra-2 Tg mice. Similarly, genetic deletion of Tgfbr2 from smooth muscle cells resulted in improvement of pulmonary vascular remodeling in the Fra-2 Tg mice, as well as a decrease in the number of Ki67-positive vascular smooth muscle cells, suggesting that TGFbeta signaling contributes to development of pulmonary vascular remodeling by promoting the proliferation of vascular smooth muscle cells. Deletion of Tgfbr2 from alpha-smooth muscle actin-expressing cells had no effect on fibrosis or inflammation in this model. These results suggest that efforts to target TGFbeta in SSc will likely require more precision than simply global inhibition of TGFbeta function. |
