Primary Identifier | MGI:2183202 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Ucp3 |
Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
Is Recombinase | false | Is Wild Type | false |
molecularNote | 4.0 kb of sequence, including the first coding exon (exon 2), was deleted and replaced with a neomycin selection cassette. Northern blot and RT-PCR analyses detected aberrantly spliced transcript, in which exon 1 had spliced directly to exon 3, in homozygous mutant mice. Protein encoded from the aberrant transcript was undetected via Western blot analysis. |