| Primary Identifier | MGI:2183246 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Nefh |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The insertion of a neomycin selection cassette deleted 1.6kb of sequence containing the proximal promoter and 34 codons, including the initiator methionine codon. Transcript was undetected in homozygous mutant mice by Northern blot analysis of RNA obtained from the brain and the spinal cord. The absence of protein was confirmed via Western blot analysis using several antibodies, capable of detecting either phosphorylated or unphosphorylated protein. |
