| Primary Identifier | MGI:2183651 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Sp4 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exons 2 and 3, encoding the amino terminal activation domains (residues 4 through 557), were replaced with a cassette containing IRES-lacZ and neo genes. Northern blot analysis of total RNA obtained from brain and heart tissue showed an absence of transcript in homozygous mutant mice. A lack of encoded protein was determined by Western blot analysis using an antibody directed against the carboxy terminal. Expression of beta-galactosidase was controlled by the promoter of the endogenous gene. |
