Primary Identifier | MGI:2183407 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | Vdr |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A floxed neo-cassette was inserted upstream of exon 2 and a third loxP site was introduced downstream of exon 2 via homologous recombination. Transient expression of cre recombinase in correctly targeted cells facilitated the removal of the neo cassette and was confirmed by Southern blot analysis. |