| Primary Identifier | MGI:2183715 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Rxrg |
| Transmission | Germline | Strain of Origin | 129S2/SvPas |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A cassette containing lacZ and neo genes was inserted into the isoform-specific exon 2. By targeting exon 2, transcription of the isoform most prominent in brain tissue is ablated, while the isoform which is produced from a transcript in which exon 1b splices to exon 3, and is most abundant in skeletal muscle, is unaffected. RT-PCR analysis confirmed that the transcript encoding the targeted isoform was absent and that the level of transcript encoding the isoform that is most prominent in skeletal muscle was normal. X-gal staining showed that beta-galactosidase is expressed via the endogenous promoter. |
