| First Author | Liu L | Year | 2020 |
| Journal | Pharmacol Res Perspect | Volume | 8 |
| Issue | 4 | Pages | e00636 |
| PubMed ID | 32776498 | Mgi Jnum | J:357820 |
| Mgi Id | MGI:7763821 | Doi | 10.1002/prp2.636 |
| Citation | Liu L, et al. (2020) Effects of RAGE inhibition on the progression of the disease in hSOD1(G93A) ALS mice. Pharmacol Res Perspect 8(4):e00636 |
| abstractText | Astrocytes play a key role in the progression of amyotrophic lateral sclerosis (ALS) by actively inducing the degeneration of motor neurons. Motor neurons isolated from receptor for advanced glycation end products (RAGE)-knockout mice are resistant to the neurotoxic signal derived from ALS-astrocytes. Here, we confirmed that in a co-culture model, the neuronal death induced by astrocytes over-expressing the ALS-linked mutant hSOD1(G93A) is prevented by the addition of the RAGE inhibitors FPS-ZM1 or RAP. These inhibitors also prevented the motor neuron death induced by spinal cord extracts from symptomatic hSOD1(G93A) mice. To evaluate the relevance of this neurotoxic mechanism in ALS pathology, we assessed the therapeutic potential of FPS-ZM1 in hSOD1(G93A) mice. FPS-ZM1 treatment significantly improved hind-limb grip strength in hSOD1(G93A) mice during the progression of the disease, reduced the expression of atrophy markers in the gastrocnemius muscle, improved the survival of large motor neurons, and reduced gliosis in the ventral horn of the spinal cord. However, we did not observe a statistically significant effect of the drug in symptoms onset nor in the survival of hSOD1(G93A) mice. Maintenance of hind-limb grip strength was also observed in hSOD1(G93A) mice with RAGE haploinsufficiency [hSOD1(G93A) ;RAGE(+/-)], further supporting the beneficial effect of RAGE inhibition on muscle function. However, no benefits were observed after complete RAGE ablation. Moreover, genetic RAGE ablation significantly shortened the median survival of hSOD1(G93A) mice. These results indicate that the advance of new therapies targeting RAGE in ALS demands a better understanding of its physiological role in a cell type/tissue-specific context. |
