| Primary Identifier | MGI:2384039 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Snrpn |
| Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<b-m2> |
| Is Recombinase | false | Is Wild Type | false |
| description | Animals carrying the mutation on a paternally derived chromosome exhibit severe growth retardation and 40%-45% die before weaning. Animals carrying the mutation on a maternally derived chromosome develop normally. |
| molecularNote | A 4.8 kb region of the gene encompassing exon 1 was replaced with a loxP site flanked neomycin resistance gene cassette. The neo cassette was removed by transient expression of cre recombinase in correctly targeted ES cells. Northern blot analysis using a probe complementary to downstream exons showed significantly reduced gene expression in animals carrying the mutation on a paternally derived chromosome, but not when the animals carried the mutation on a maternally derived chromosome. |
