| Primary Identifier | MGI:2384535 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Cckbr |
| Transmission | Germline | Strain of Origin | 129S4/SvJae |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Part of exon 2 and exons 3-5 were replaced with a lacZ-PGK-neo cassette via homologous recombination resulting in deletion of the majority of 7 transmembrane regions after amino acid 108. Absence of gene expression was demonstrated by Northern blot analysis of cerebral cortex, basal ganglion, stomach, and pancreas mRNA from homozygous mutant animals using a 2.3 kb cDNA fragment as the probe. Absence of functional receptor protein was confirmed via binding assays using brain cell membrane preparations from homozygous mutants. |
