Primary Identifier | MGI:2183934 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Rad54l |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A neomycin selection cassette was inserted into exon 4, upstream of approximately 90% of the protein product including the conserved motifs involved in ATPase activity. Sequence analysis showed that excision of the disrupted exon would result in a frameshift mutation. The absence of transcript and protein was determined by Northern and Western blot analysis. |