| First Author | Taccioli GE | Year | 1998 |
| Journal | Immunity | Volume | 9 |
| Issue | 3 | Pages | 355-66 |
| PubMed ID | 9768755 | Mgi Jnum | J:50222 |
| Mgi Id | MGI:1290043 | Doi | 10.1016/s1074-7613(00)80618-4 |
| Citation | Taccioli GE, et al. (1998) Targeted disruption of the catalytic subunit of the DNA-PK gene in mice confers severe combined immunodeficiency and radiosensitivity. Immunity 9(3):355-66 |
| abstractText | The DNA-dependent protein kinase is a mammalian protein complex composed of Ku70, Ku80, and DNA-PKcs subunits that has been implicated in DNA double-strand break repair and V(D)J recombination. Here, by gene targeting, we have constructed a mouse with a disruption in the kinase domain of DNA-PKcs, generating an animal model completely devoid of DNA-PK activity. Our results demonstrate that DNA-PK activity is required for coding but not for signal join formation in mice. Although our DNA-PKcs defective mice closely resemble Scid mice, they differ by having elevated numbers of CD4(+)CD8(+) thymocytes. This suggests that the Scid mice may not represent a null phenotype and may retain some residual DNA-PKcs function. |
