| Primary Identifier | MGI:2384158 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Pcmt1 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exons 2 and 3 were replaced with a neomycin selection cassette. The deleted region encoded amino acids 19 through 64. The absence of transcript and protein was determined by RNAse protection and Western blot analyses of homozygous mutant brains and testes. Methyltransferase activity assays showed activity to be ablated in lysates of brain, testis, and eye tissue and reduced in renal lysates. Other enzymes were believed to be responsible for the activity observed in the kidney. |
