| First Author | Okuyama K | Year | 2012 |
| Journal | Biochem Biophys Res Commun | Volume | 417 |
| Issue | 1 | Pages | 141-6 |
| PubMed ID | 22138652 | Mgi Jnum | J:180314 |
| Mgi Id | MGI:5306089 | Doi | 10.1016/j.bbrc.2011.11.072 |
| Citation | Okuyama K, et al. (2012) A checkpoint in B-lymphopoiesis related to Notch resistance. Biochem Biophys Res Commun 417(1):141-6 |
| abstractText | While murine B- and T-lymphopoiesis require overlapping molecules, they occur in separate organs: the bone marrow (BM) and the thymus, respectively. The BM microenvironment is incapable of supporting T-lymphopoiesis because of insufficient interactions of Notch1 with delta-like ligand (Dll). Notch1/Dll interactions also play a role in the suppression of B-lymphopoiesis in the thymus. However, it is still unclear whether the Notch1/Dll interaction alone explains why the thymus does not support B-lymphopoiesis. In this study, we compared the precursor population colonizing the thymus with that in the BM by culturing them on stromal cells expressing abundant Dll1. We demonstrated that Flt3(+) Il7r(+) B220(+) Cd19(+) BM cells gave rise to B cells under this condition. We defined them as resistant to Dll1. In the thymus, Dll1-resistant cells were undetectable. This suggested that the absence of Dll1-resistant cells might explain the absence of B-lymphopoiesis in the thymus. |
