| Primary Identifier | MGI:2384127 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Plp1 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | 105 bp of exon 3 were deleted to mimic the DM20 isoform and a neomycin resistance cassette was inserted into intron 3 to disrupt splicing of the gene product. Northern blot analysis confirmed the absence of normal transcripts in the brain of homozygous mutant animals. Western blot analysis and silver stained SDS-PAGE gels of myelin samples from homozygous mutants did not detect protein product of either isoform, PLP or DM20. |
