| First Author | Stanley AC | Year | 2012 |
| Journal | J Leukoc Biol | Volume | 92 |
| Issue | 6 | Pages | 1227-39 |
| PubMed ID | 23012430 | Mgi Jnum | J:191106 |
| Mgi Id | MGI:5460956 | Doi | 10.1189/jlb.0412191 |
| Citation | Stanley AC, et al. (2012) Recycling endosome-dependent and -independent mechanisms for IL-10 secretion in LPS-activated macrophages. J Leukoc Biol 92(6):1227-39 |
| abstractText | IL-10 is a key anti-inflammatory cytokine secreted by activated macrophages as a feedback control mechanism to prevent excessive inflammatory responses. Here, we define multiple intracellular trafficking pathways involved in the secretion of newly synthesized IL-10 from macrophages following TLR4 activation with LPS, and show how this relates to the previously defined trafficking pathways for IL-6 and TNF in macrophages simultaneously producing these proinflammatory cytokines. IL-10 exits the Golgi in multiple tubular carriers, including those dependent on p230GRIP. Some of the IL-10 is then delivered to recycling endosomes, where cytokine sorting may occur prior to its release. Another portion of the IL-10 is delivered to the cell surface in distinct vesicles colabeled for apoE. Thus, we show at least two post-Golgi pathways via which IL-10 is trafficked, ensuring its secretion from activated macrophages under different physiological conditions. |
