| Primary Identifier | MGI:2385728 | Allele Type | Targeted |
| Gene | Lhx1 | Transmission | Germline |
| Strain of Origin | 129S7/SvEvBrd-Hprt1<+> | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The neo cassette inserted into intron 1 of Lhxtm3Bhr was excised by breeding with CMV-cre transgenic mice. The four missense mutations affecting conserved cysteine residues in exons 1 and 2, encoding the two LIM domains, were left intact. The conservation of the endogenous expression pattern was verified by whole mount in situ hybridization of homozygous mutant embryos. RT-PCR and sequence analyses showed normal expression and the exclusive presence of the missense mutations. Finally, immunocytochemical analysis confirmed the presence of protein in mutant mice. |
