| First Author | Murugan S | Year | 2012 |
| Journal | Genesis | Volume | 50 |
| Issue | 9 | Pages | 693-9 |
| PubMed ID | 22467513 | Mgi Jnum | J:187859 |
| Mgi Id | MGI:5438460 | Doi | 10.1002/dvg.22028 |
| Citation | Murugan S, et al. (2012) Conditional expression of Lodavin, an avidin-tagged LDL receptor, for biotin-mediated applications in vivo. Genesis 50(9):693-9 |
| abstractText | Lodavin represents an engineered fusion protein that consists of a cytoplasmic and a transmembrane domain of the human low-density lipoprotein receptor coupled to an extracellular avidin monomer. Biotinylated compounds have been successfully targeted to Lodavin-expressing cells that have been transduced by a Lodavin-containing virus, and the targeting is based on the high affinity between biotin and avidin. We engineered a Rosa26 (R26R) knock-in Lodavin mouse to develop biotin-based applications such as targeted drug delivery, cell purification, and tissue imaging in vivo. A cDNA encoding Lodavin was inserted downstream of a floxed betageo resistance gene in the R26R locus in embryonic stem cells, and a germ line-derived R26RLodavin mouse line was generated. Efficient removal of the floxed betageo cassette and conditional activation of Lodavin expression was achieved as a result of crossing the R26RLodavin mice with HoxB7-Cre, Wnt4-Cre, or Tie1-Cre mice. In summary, the R26RLodavin mouse line may provide a useful tool for testing and developing applications with the aid of avidin and biotin interaction. genesis 50:693-699, 2012. (c) 2012 Wiley Periodicals, Inc. |
