| Primary Identifier | MGI:2386167 | Allele Type | Targeted |
| Attribute String | Hypomorph | Gene | Fli1 |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| description | TC1-10 ES cells were used. |
| molecularNote | A floxed neo cassette was inserted into exon 9 via homologous recombination resulting in a premature stop codon between the DNA-binding domain and the CTA domain. The targeted mutation results in a hypomorphic allele. Northern blot analysis of homozygous mutant embryos showed 2 gene transcripts at 10% the level of wild-type transcription. Western blot analysis detected a truncated protein expressed at very low levels. Enzyme assay determined that the truncated protein exhibits 50%-60% of the activity of normal protein. |
