| Primary Identifier | MGI:2386404 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Spam1 |
| Transmission | Germline | Strain of Origin | 129S2/SvPas |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Part of exon 2, which encodes the hyaluronidase domain, was replaced with a neomycin resistance cassette via homologous recombination. Testicular RNA from homozygous mutant animals did not contain gene transcripts as assayed by Northern blot. Western blot of cauda epididymal sperm protein extracts from homozygous mutants showed no immunoreactivity. |
