| Primary Identifier | MGI:2386439 | Allele Type | Targeted |
| Attribute String | Hypomorph, Null/knockout | Gene | Myh10 |
| Transmission | Germline | Strain of Origin | 129S4/SvJae |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A 30 bp neural-specific exon referred to as N30 (also called the B1 insert) and the flanking introns were replaced with a PGK-neo cassette via homologous recombination. Deletion of the B1 insert was confirmed by RNA analysis of neural tissues. Northern blot of various tissues from homozygous mutant mice showed a significant decrease in gene expression. Western blot analysis showed a significant decrease in protein expression in both embryonic and adult mutant mice, including a decrease of 88% in the heart, of 65% in the brain, and of 64% in the lung. This is a mildly hypomorphic allele. |
