| First Author | Fu G | Year | 2012 |
| Journal | J Immunol | Volume | 189 |
| Issue | 5 | Pages | 2326-32 |
| PubMed ID | 22837484 | Mgi Jnum | J:189870 |
| Mgi Id | MGI:5447144 | Doi | 10.4049/jimmunol.1103458 |
| Citation | Fu G, et al. (2012) Phospholipase Cgamma2 plays a role in TCR signal transduction and T cell selection. J Immunol 189(5):2326-32 |
| abstractText | One of the important signaling events following TCR engagement is activation of phospholipase Cgamma (PLCgamma). PLCgamma has two isoforms, PLCgamma1 and PLCgamma2. It is known that PLCgamma1 is important for TCR signaling and TCR-mediated T cell selection and functions, whereas PLCgamma2 is critical for BCR signal transduction and BCR-mediated B cell maturation and functions. In this study, we report that PLCgamma2 was expressed in primary T cells, and became associated with linker for activated T cells and Src homology 2-domain containing leukocyte protein of 76 kDa and activated upon TCR stimulation. PLCgamma1/PLCgamma2 double-deficient T cells displayed further block from CD4 and CD8 double-positive to single-positive transition compared with PLCgamma1 single-deficient T cells. TCR-mediated proliferation was further impaired in PLCgamma1/PLCgamma2 double-deficient T cells compared with PLCgamma1 single-deficient T cells. TCR-mediated signal transduction, including Ca(2)(+) mobilization and Erk activation, was further impaired in PLCgamma1/PLCgamma2 double-deficient relative to PLCgamma1 single-deficient T cells. In addition, in HY TCR transgenic mouse model, thymic positive and negative selections were reduced in PLCgamma1 heterozygous- and PLCgamma2 homozygous-deficient (PLCgamma1(+)/(-)PLCgamma2(-)/(-)) relative to wild-type, PLCgamma2 single-deficient (PLCgamma2(-)/(-)), or PLCgamma1 heterozygous-deficient (PLCgamma1(+)/(-)) mice. Taken together, these data demonstrate that PLCgamma2 participates in TCR signal transduction and plays a role in T cell selection. |
