Primary Identifier | MGI:2386272 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Chuk |
Transmission | Germline | Strain of Origin | 129S4/SvJae |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A 3 kb region containing the promoter, exon 1, and part of intron 1 was replaced with a PGK-neo cassette via homologous recombination. The null allele was confirmed by Western blot analysis performed on primary embryonic fibroblasts cultured from homozygous mutant embryos. No protein expression was detected. |