| First Author | Omilusik KD | Year | 2021 |
| Journal | J Immunol | Volume | 207 |
| Issue | 5 | Pages | 1377-1387 |
| PubMed ID | 34380645 | Mgi Jnum | J:309127 |
| Mgi Id | MGI:6756255 | Doi | 10.4049/jimmunol.2100303 |
| Citation | Omilusik KD, et al. (2021) Ubiquitin Specific Protease 1 Expression and Function in T Cell Immunity. J Immunol 207(5):1377-1387 |
| abstractText | T cells are essential mediators of immune responses against infectious diseases and provide long-lived protection from reinfection. The differentiation of naive to effector T cells and the subsequent differentiation and persistence of memory T cell populations in response to infection is a highly regulated process. E protein transcription factors and their inhibitors, Id proteins, are important regulators of both CD4(+) and CD8(+) T cell responses; however, their regulation at the protein level has not been explored. Recently, the deubiquitinase USP1 was shown to stabilize Id2 and modulate cellular differentiation in osteosarcomas. In this study, we investigated a role for Usp1 in posttranslational control of Id2 and Id3 in murine T cells. We show that Usp1 was upregulated in T cells following activation in vitro or following infection in vivo, and the extent of Usp1 expression correlated with the degree of T cell expansion. Usp1 directly interacted with Id2 and Id3 following T cell activation. However, Usp1 deficiency did not impact Id protein abundance in effector T cells or alter effector T cell expansion or differentiation following a primary infection. Usp1 deficiency resulted in a gradual loss of memory CD8(+) T cells over time and reduced Id2 protein levels and proliferation of effector CD8(+) T cell following reinfection. Together, these results identify Usp1 as a player in modulating recall responses at the protein level and highlight differences in regulation of T cell responses between primary and subsequent infection encounters. Finally, our observations reveal differential regulation of Id2/3 proteins between immune versus nonimmune cell types. |
