| First Author | Essig K | Year | 2018 |
| Journal | Nat Commun | Volume | 9 |
| Issue | 1 | Pages | 3810 |
| PubMed ID | 30232334 | Mgi Jnum | J:267623 |
| Mgi Id | MGI:6267780 | Doi | 10.1038/s41467-018-06184-3 |
| Citation | Essig K, et al. (2018) Roquin targets mRNAs in a 3'-UTR-specific manner by different modes of regulation. Nat Commun 9(1):3810 |
| abstractText | The RNA-binding proteins Roquin-1 and Roquin-2 redundantly control gene expression and cell-fate decisions. Here, we show that Roquin not only interacts with stem-loop structures, but also with a linear sequence element present in about half of its targets. Comprehensive analysis of a minimal response element of the Nfkbid 3'-UTR shows that six stem-loop structures cooperate to exert robust and profound post-transcriptional regulation. Only binding of multiple Roquin proteins to several stem-loops exerts full repression, which redundantly involved deadenylation and decapping, but also translational inhibition. Globally, most Roquin targets are regulated by mRNA decay, whereas a small subset, including the Nfat5 mRNA, with more binding sites in their 3'-UTRs, are also subject to translational inhibition. These findings provide insights into how the robustness and magnitude of Roquin-mediated regulation is encoded in complex cis-elements. |
